Conformational and Linear Epitope Mapping Services
CovalX provides various techniques for mapping of conformational epitopes using the most advanced mass spectrometry equipment.
Crosslinking Coupled Mass Spectrometry (XL-MS)
Based on our unique MALDI mass spectrometry technology and decades of crosslinking experiences, CovalX offers characterization of epitopes with high resolution. Using mass spectrometry and covalent tagging it is possible to characterize conformational and linear epitopes in weeks, with a resolutions of 1-3 amino acids. No need for peptide synthesis or micro-array chips, mass spectrometry allows identity of the interacting peptides with unmatched accuracy.
This technique provides the lowest cost, lowest sample consumption and shortest analysis time possible when compared with other conformational epitope mapping technique available. Additionally because the complexes are first covalently stabilized, multiple enzymes can be utilized providing highly optimized digestion conditions with the highest sequence coverage possible.
This technique can provide both epitope and paratope data in one experiment. Over the years this technique has been established as a reliable source of data for both research and patent filing purposes.
Hydrogen Deuterium Exchange (HDX)
HDX has been utilized on a research scale for many years at the academic level and within larger pharmaceutical companies. More recently with the advancement of automated robotics, sample handling and data analysis software, HDX can now be offered on a timescale and with sample consumptions reasonable for more mainstream analysis.
CovalX is using the latest mass spectrometry equipment, HDX automation robotics and software to rapidly characterize the binding sites using HDXMS. Additionally, CovalX has technicians with combined decades of experience optimizing and understanding HDXMS workflows.
-Speed: 3-4 weeks for one epitope; 5-6 weeks for projects with up to 5 epitopes against the same antigen. Possibility to screen simultaneously up to 100 monoclonal antibody against the same target.
-Sample Consumption: 150 ug of antigen and 150 ug of mAb/epitope.
-Resolution down to 1-3 amino-acids.
-Applicable for conformational, discontinuous and linear epitopes.
-Applicable for any therapeutic proteins or antigen. No limit in size. No limit in complexity (multimers, hydrophobicity)
-No need for crystallography, recombinant protein work, peptide synthesis, micro-array. The interaction is analyzed directly, by mass spectrometry.
-Cost: The CovalX techniques save money in 3 ways
1: Lowest cost for analysis of any true conformational technique
2: Sample consumption: Lowest sample used saves on production costs/time
3: Time: Our techniques are fast and reliable.