The ability of an antigen to simultaneously bind two different monoclonal antibodies needs to be addressed for the structural characterization of both antigens and monoclonal antibodies. In practice, this is important for the development of immunochemical sandwich assays. By combining chemical cross-linking and High-Mass MALDI mass spectrometry, it becomes possible to perform multibinding experiments with high accuary and sensitivity.
Multibinding experiments analyzed by CovalX HM1 High-Mass System. Two monoclonal antibodies against the prion protein 6H4 and 3B8 are incubated in the presence of the bovine prion protein. The complex 6H4-2bPrP-3B8 is observed at m/z= 356 kDa.
Bich et al. Anal. Biochem. 2008, 375:35-45.