Protein Complex Analysis
Using cross-linking chemistry and High-Mass MALDI ToF mass spectrometry, it becomes possible to easily analyze non-covalent protein complexes in the 1-1000 kDa range.
The use of High-Mass MALDI allows a much faster, cheaper and more accurate method for studying protein interactions:
- Stoechiometry of the complex: unique information for understanding the shape of your protein complex.
- Clear identification of the partners involved in the complex.
- No need for immobilization, the complex is detected without labeling.
- Fast: 30 minutes per assay.
- Specific: only the specific complexes are detected.
- Sensitive: Only a few hundred femtomol of protein sample is a few ul are require to achieve the analysis.
In the spectrum presented, the protein complex AMPk is analyzed by High-Mass MALDI ToF mass spectrometry before cross-linking (black) and after corss-linking (red) with CovalX's K200 MALDI MS Analysis kit. Before cross-linking, the three subunits α, β and γ of the protein complex are detected with m=30.22 kDa, m=37.38 kDa and m=64.97 kDa. After cross-linking, the intact protein complex corresponding to the stoechionetry [α·β·γ] is detected with m=129.95 kDa. Riek U et al.; J. Biol. Chem.; 2008."; ?>