Patents, Publications and Presentations



Patents filed using CovalX's technology

  • POLYMERIC IgA-TYPE RECOMBINANT ANTIBODY AND USE THEREOF. Shinji Saito et al., Japanese National Institute Of Infectious Diseases (Japan)Publication Date: 01/21/2016. more.
  • ANTIBODIES TO CANINE INTERLEUKIN-4 RECEPTOR ALPHA. Morsey, Mohamad et al., Intervet International B.V. (Summit, New Jersey, US)Publication Date: 10/06/2016. more.
  • METHODS TO SELECT FOR AGENTS THAT STABILIZE PROTEIN COMPLEXES. Steyaert, Jan et al., Vrije Universiteit Brussel (Brussel, BE)Publication Date: 01/28/2016. more.
  • MK2 INHIBITORS AND USES THEREOF. Alexander, Matthew David et al., Celgene Avilomics Research, Inc. (Bedford, MA, US)Publication Date: 12/31/2015. more.
  • IL-15R ALPHA FORMS, CELLS EXPRESSING IL-15R ALPHA FORMS, AND THERAPEUTIC USES OF IL-15R ALPHA AND IL-15/IL-15R ALPHA COMPLEXES. BK Felber, S Finkielsztein, GN Pavlakis, JN Vournakis, Admune Therapeutics LLC (Danvers, MA, US), USA Dept. of Health and Human Services (Bethesda, MD, US)Published: 12/17/2015. more.
  • METHOD FOR TREATING A DISEASE ASSOCIATED WITH SOLUBLE, OLIGOMERIC SPECIES OF AMYLOID BETA 1-42. Goure, William F., et al.  Acumen Pharmaceuticals, INC., Merck Sharp & Dohme Corp.Published: 11/12/2015. more.
  • METHODS AND COMPOSITIONS FOR SCREENING AND DETECTING BIOMARKERS. Jordan, Nancy Tommye Ann (Knoxville, TN, US), EDP Biotech Corporation (Knoxville, TN, US)Published: 10/29/2015. more.
  • METHOD FOR NEUTRALIZING HEPATITIS C VIRUS, FULLY HUMAN MONOCLONAL ANTIBODY AGAINST HEPATITIS C VIRUS (VARIANTS), COMPOSITION OF FULLY HUMAN MONOCLONAL ANTIBODIES AGAINST HEPATITIS C VIRUS, AND HYBRID MOUSE/HUMAN PRODUCER CELL LINE OF FULLY HUMAN MONOCLONAL ANTIBODIES AGAINST HEPATITIS C VIRUS (VARIANTS). Vlasik, Tatiana Nikolaevna, et al., Published: 07/02/2015. more.
  • ANTIBODIES AGAINST CANINE PD-1. Morsey, Mohamad et al., Intervet Inc. (Summit, New Jersey, US)Publication Date: 06/25/2015. more.
  • CANINIZED ANTIBODIES. Morsey, Mohamad et al., Intervet Inc. (Summit, New Jersey, US)Publication Date: 06/25/2015. more.
  • METHODS OF TREATMENT FOR ALZHEIMER'S DISEASE AND HUNTINGTON'S DISEASE. Rosenthal, Arnon et al., Annexon, Inc. (South San Francisco, CA, US)and Boston Children's Hospital (Boston, MA, US)Publication Date: 01/15/2015. more.
  • ANTI-COMPLEMENT FACTOR C1Q ANTIBODIES AND USES THEREOF. Rosenthal, Arnon and Leviten, Michael, Annexon, Inc. (South San Francisco, CA, US) Publication Date: 01/15/2015. more.
  • METHODS OF TREATMENT FOR GUILLAIN-BARRE SYNDROME. Rosenthal, Arnon et al., Annexon, Inc. (South San Francisco, CA, US) and The University Court of the University of Glasgow(Glasgow, GB) Publication Date: 11/20/2014. more.
  • CAPSULAR GRAM-POSITIVE BACTERIA BIOCONJUGATE VACCINES. Wacker, Michael et al., Glycovaxyn AG (Schlieren, CH)Publication Date: 10/28/2014. more.
  • BINDING PROTEINS COMPRISING AT LEAST TWO REPEAT DOMAINS AGAINST HER2. Fiedler, Ulrike, et al., Molecular Partners AG (Zurich-Schlieren, CH)Published 06/05/2014. more.
  • METHODS FOR ISOLATING AND QUANTIFYING ANTIGEN FROM VACCINES. Graninger, Michael and Kaliwoda, Martin, Baxter International Inc.Publication Date: 10/26/2012. more.
  • BIOPOLYMER, IMPLANT COMPRISING IT AND USES THEREOF. Rodríguez Cabello, José Carlos et al., Universidad, de Valladolid (Valladolid, ES)Publication Date: 12/21/2011. more.
  • MALDI Mass Spectrometry (MALDI-MS) for proteins of high molecular weight. Fournier, Isabelle, et al., Centre National de la Recherche Scientifique (CNRS) (Paris, FR)Published: 05/04/2011. more.

Peer Reviewed Scientific Articles using CovalX's technology

2017

  • Synthetic toxic Aβ1–42 oligomers can assemble in different morphologies. Bobo et al., Biochimica et Biophysica Acta (BBA) - General SubjectsVolume 1861, Issue 5, Part A, May 2017, Pages 1168–1176. more.

 

2016

  • Recombinant phospholipase A1 of the outer membrane of psychrotrophic Yersinia pseudotuberculosis: Expression, purification, and characterization. S. I. Bakholdina et al., Biochemistry (Moscow)02 February 2016. more.
  • Polymer-based metal nano-coated disposable target for matrix-assisted and matrix-free laser desorption/ionization mass spectrometry. S. Bugovsky et al., MethodsVolume 104, 15 July 2016, Pages 182–193. more.
  • Structural characterization of the yeast CF IA complex through a combination of mass spectrometry approaches. Stojko et al., International Journal of Mass SpectrometryAvailable online 17 August 2016. more.
  • Microarray-based MALDI-TOF mass spectrometry enables monitoring of monoclonal antibody production in batch and perfusion cell cultures. Steinhoff et al., MethodsVolume 104, 15 July 2016, Pages 33–40. more.
  • Adaptive responses of outer membrane porin balance of Yersinia ruckeri under different incubation temperature, osmolarity, and oxygen availability. Bystritskaya et al., Microbiology Open1 April 2016. more.
  • Capitalizing Resolving Power of Density Gradient Ultracentrifugation by Freezing and Precisely Slicing Centrifuged Solution: Enabling Identification of Complex Proteins from Mitochondria by Matrix Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry. Yu et al., Journal of Analytical Methods in ChemistryAccepted 7 August 2016. more.
  • How human IgGs against DNA recognize oligonucleotides and DNA. Andreev et al., Journal of Molecular Recognition25 August 2016. more.
  • Structural basis of transcobalamin recognition by human CD320 receptor. Alam et al., Nature CommunicationsPublished online: 14 July 2016. more.

 

2015

  • Microarray-based MALDI-TOF mass spectrometry enables monitoring of monoclonal antibody production in batch and perfusion cell cultures. Steinhoff et al., Methods, Available Online: 18 December 2015. more.
  • Internalized Tau Oligomers Cause Neurodegeneration by Inducing Accumulation of Pathogenic Tau in Human Neurons Derived from Induced Pluripotent Stem Cells. Usenovic, et al., The Journal of Neuroscience 21 October 2015, 35(42): 14234-14250. more.
  • Structure and flexibility of the endosomal Vps34 complex reveals the basis of its function on membranes. Rostislavleva et al., Science09 Oct 2015: Vol. 350, Issue 6257, pp. more.
  • Structure of a PEGylated protein reveals a highly porous double-helical assembly. Cattani et al., Nature Chemistry7, 823–828 (2015). more.
  • Analysis of a Common Cold Virus and Its Subviral Particles by Gas-Phase Electrophoretic Mobility Molecular Analysis and Native Mass Spectrometry. Weiss et al., Anal. Chem.2015, 87 (17), pp 8709–8717. more.
  • Hsp90 oligomers interacting with the Aha1 covhaperone: An outlook for the Hsp90 chaperone machineries. Lepvrier et al., Anal. Chem2015 Jul 21;87(14):7043-51. more.
  • Improved analysis of ultra-high molecular mass polystyrenes in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using DCTB matrix and caesium salts. Gabriel SJ et al., Rapid Comm Mass Spec2015 Jun 15;29(11):1039-46. more.
  • Hsp90 oligomerization process: How can p23 drive the chaperone machineries? Lepvrier et al., BBA, Vol 1854, Issue 10, Part A, October 2015, p 1414-1424. more.
  • Recent advances in the characterization of hair of mummies from the Chilean Andean coast. Fresnais et al., Forensic Science InternationalApril 2015, Volume 249, 25-34. more.
  • Chemical cross-linking and mass spectrometry to determine the subunit interaction network in a recombinant human SAGA HAT subcomplex. Nguyen-Huynh et al., Protein Science, 9 March 2015, epub. more.
  • Quantitative analysis of PEG-functionalized colloid gold nanoparticles using charged aerosol detection. Smith et al., Analytical and Bioanalytical Chemistry, 8 March 2015, epub. more.
  • On the Efficiency of NHS Ester Cross-Linkers for Stabilizing Integral Membrane Protein Complexes. Chen et al., Journal of the American Society of Mass Spectrometry, March 2015, Volume 26, Issue 3, 493-498. more.
  • Development and Vaildation of an Immuno-PET Tracer as a Commpanion Diagnosti Agent for Antibody-Drug Conjugate Therapy to Target the CA6 Epitope. Ilovich et al., Radiology, 27 February 2015, epub. more.
  • Human placenta: relative content of antibodies of different classes and subclasses (IgG1-IgG4) containing lambda- and kappa-light chains, and chimeric lambda-kappa-immunoglobulins. Lekchnov et al., International Immunology, 1 February 2015, epub. more.
  • Very stable high molecular mass multiprotein complex with DNase and amylase activities in human milk. Soboleva SE et al., J Mol RecognitionVolume 28, Issue 1, pages 20–34, January 2015. more.


2014

  • Extremely Stable Soluble High Molecular Mass Multi-Protein Complex with DNase Activity in Human Placental Tissue. Burkova et al., PLUS One, 26 November 2014, Volume 9, Issue 11, e111234. more.
  • High-Mass MALDI-MS Using Ion Conversion Dynode Detectors: Influence of the Conversion Voltage on Sensitivity and Spectral Quality. Weidmann & Zenobi, Journal of the American Society of Mass Spectrometry, June 2014, Volume 25, Issue 6, 950-954. more.
  • Gene disruption by cell-penetrating peptide-mediated delivery of Cas9 protein and guide RNA. Ramakrishna et al., Genome Research, April 2014, Volume 24, Page 1020-1027. more.
  • An integrated cross-linking-MS approach to
    investigate cell penetrating peptides interacting
    partners
    An integrated cross-linking-MS approach toinvestigate cell penetrating peptides interacting partners. Clavier et al., EuPA Open Proteomics, March 2014, Volume 3, Page 229-238
    . 
    more.


2013

  • Direct access to aptamer–protein complexes via MALDI-MS. Chen et al., Chemical Sciences, October 2013, Volume 4, Issue 10, 4071-4078. more...
  • Optimized Protocol for Protein Macrocomplexes Stabilization Using the EDC, 1-Ethyl-3-(3-(dimethylamino)propyl)carbodiimide, Zero-Length Cross-Linker. Lepvrier et al., Analytical Chemistry, 30 September 2013, Volume 86, Issue 21, 10524-10530. more...
  • Mass Discrimination in High-Mass MALDI-MS. Wiedmann et al., Journal of the American Society for Mass Spectrometry, September 2013, Volume 24, Issue 9, 1396-1404. more...
  • Structural and Functional Role of INI1 and LEDGF in the HIV-1 Preintegration Complex. Maillot et al., PLOS ONE, April 2013, Volume 8, Issue 4, e60734. more...
  • Characterization and Favorable in Vivo Properties of heterodimeric soluble IL-15-IL-15Ralfa Cytokine Compared to IL-15 monomer. Chertova et al., J. Biol. Chem, 2013, 288:18093-18103. more..
  • A New, Modular Mass Calibrant for High-Mass MALDI-MS. Weidmann et al., Analytical Chemistry, 2013, 85, 3425-3432. more...
  • High-Mass Matrix-Assisted Laser Desorption Ionization-Mass Spectrometry of Integral Membrane Proteins and Their Complexes. Chen, F et al., Anal. Chem., 2013, 85 (7), 3483–3488. more...
  • Understanding chemical reactivity for homo and heterobifunctional protein cross-linking agents. Chen, F et al., J. Mass. Spectrom. 2013, 48,807-812. more...
  • Studying the fate of non-volatile organic compounds in a commercial plasma air purifier. Schmid, S et al., Journal of Hazardous Material, 2013, 256-257; 76-83. more...
  • Detection systems for mass spectrometry imaging: A perspective on novel developments with a focus on active pixel detectors. Jungmann and Heeren, Rapid Communicaion in Mass Spectrometry, January 2013, Volume 27, Issue 1, 1-23. more...


2012

  • Microship capillary gel electrophoresis of multiply PEGylated high-molecular-mass glycoprotein. Seyfried, B et al., Biotechnology Journal, 2012; Vol 7, Issue 5:635-641.  more...
  • Monitoring ligand modulation of protein-protein interactions by chemical cross-linking and High-Mass MALDI mass spectrometry. Gasilova, N et al., Methods Mol. Biol., 2012; 803:219-29. more...
  • Molecular Cloning, Isolation, and Properties of Chaperone Skp from Yersinia pseudotuberculosis. Sidorin, E, V et al., Biochemistry2012; Vol 77, Issue 11:1315-1325.  more...
  • A novel mechanism of iron-core formation by Pyrococcus Furiosus archaeoferrtin, a member of an uncharacterized branch of the ferritin like superfamily. Ebrahimi, K et al., J. Biol. Inorg. Chem2012; 17:975-985.  more...
  • Characterization of thermostable deblocking aminopeptidases of archaeon Thermococcus annurineus NA1 by proteomic and biochemical approches. Lee, YG et al., Journal of Microbiology. 2012; Vol 50, Issue 5 p792-797.  more...
  • MALDI-MS detection of noncovalent interactions of single stranded DNA with Escherichia coli single-straded DNA-binding protein. Chen, F et al., J. Mass. Spectrom, 2012, 47, 560-566. more...
  • MIRG Survey2011:Snapshot of Rapidly Evolving Label-Free Technologies Used for characterizing Molecular Interactions. Yadav, S.P. et al., JBT, 2012, 23:94-100. more...


2011

  • Compelling Advantages of Negative Ion Mode Detection in High-Mass MALDI-MS for Homomeric Protein Complexes. Madler, S et al., JASMS, 2012, 23(2), 213-224. more...
  • A Specific Protein Disorder Catalyser of HIV-1 Nef. Lugari, A et al., Bioorganic & Medicinal Chemistry, 2011, 19, 7401-7406. more...
  • The Structural Basis For Increased Toxicity of Pathological AB42:AB40 Ratios in Alzheimer's Disease. Pauwels, K et al., Journal of Biological Chemistry, 2011, jbcM111.264473. more...


2010

  • Does Chemical Cross-Linking with NHS Esters Reflect the Chemical Equilibrium of Protein-Protein Noncovalent Interactions in Solution? Mädler, S et al., JASMS; 2010, Vol 21, No. 10, pp 1775-1783. more...
  • MALDI imaging and profiling MS of higher mass proteins from tissue. van Remoortere, A et al. JASMS; 2010, Vol 21, No. 11, pp 1922-1929. more...
  • MALDI linear TOF mass spectrometry of PEGylated (glyco)proteins. Seyfried, B et al. Journal of Mass Spectrometry, 2010, Vol. 46, Iss. 6, pp 612-617, June 2010. more...
  • Detection of Nucleic Acid-Nuclear Hormone Receptor complexes with mass spectrometry. Bich, C et al. JASMS; 2010, 21, 635-645. more...
  • Biochemical and Biophysical characterization of the Mg2+ induced 90 kDa heat shock protein oligomers. Moullintraffort, L et al. Journal Biological Chemistry, 2010, JBC, May 14th 2010. more...
  • Reactivity and Applications of New Amine Reactive Cross-Linker for Mass Spectrometry Detection of Protein Complexes. Bich, C et al. Anal. Chem., 2010, 82 (1), pp 172–179. more...
  • Probing the Hydrophobic Effect of Noncovalent Complexes by Mass Spectrometry. Bich, C et al. JASMS, 2010, 21(2) 286-289. more...


2009

  • Mass spectrometry of large complexes. Bich, C. and R. Zenobi, Current Opinion in Structural Biology, 2009, 19, 1-8. more...
  • Characterization of high molecular weight multimeric states of human haptoglobin and hemoglobin-based oxigen carriers by high-mass MALDI MS. Pimenova, T. et al. J. Sep. Sci. 2009, 32, 1224-1230. more...
  • Towards high-throughput identification of endocrine disrupting compounds with mass spectrometry. Bovet, C. et al. Toxicology in Vitro; 2009, 23, 4, 704-709. more...
  • Structural basis for HIV-1 DNA integration in the human genome, role of the LEDGF/P75 cofactor. Michel F et al. The EMBO Journal; 2009, 28, 980-991. more...


2008

  • The reaction of hydrogen peroxide with hemoglobin induces extensive alpha-globin crosslinking and impairs the interaction of hemoglobin with endogenous scavenger pathways. Vallelian F et al. Free Radical & Medicine 45 (2008) 1150-1158. more...
  • Monitoring Ligand Modulation of Protein-Protein Interactions by Mass Spectrometry: Estrogen Receptor r-SRC1. Bovet C. et al. Anal. Chem., 80, (2008), 7833–7839. more...
  • Structural properties of AMP-activated protein kinase. Dimerization, molecular shape, and changes upon ligand binding.  Riek U et al. J. Biol. Chem; June 27 2008; Vol. 283, No. 26, pp. 18331–18343, more...
  • Structural Characterization of human general transcription factor TFIIF in solution. Akashi S et al. Protein Science; 2008; 17: 389–400. doi: 10.1110/ps.073258108. more...
  • Characterization of antibody-antigen interactions: Comparison between surface plasmon resonance measurements and high-mass matrix assisted laser desorption/ionization mass spectrometry. Bich C et al. Anal. Biochem.; 2008; 375:35-45. more...
  • Epitope mapping on bovine prion protein using chemical cross-linking and mass spectrometry. Pimenova et al. J. Mass Spectrom.; 2008; 43:185-195. more...
  • Proteomic Profiling of a Snake Venom Using High Mass Detection MALDI-TOF Mass Spectrometry - Yanes O et al. JASMS; 2007; 18(4); 600-606. more...
  • Detection of non-covalent complexes in biological samples by intensity fading and high-mass detection MALDI ToF mass spectrometry. Yanes O et al. J. Proteom. Research; 2006; 5:10; 2711-2719. more...
  • Immunoassays with direct mass spectrometric detection. Nazabal et al. Anal. Chem.; 2006; 78(11); 3562-3570. more...

 

Selected Oral and Poster Conference Presentations

ASMS 2012 Conference, May 20-24, 2012, Vancouver, BC, Canada
 Enhancement of high mass sensitivity on the Bruker UltraFlextreme with FlashDetector technology using the CovalX HM2. Ryan Wenzel, Young-Ho Chung, Alexis Nazabal

 Therapeutic Protein Aggregates: Direct Characterization and Quantitation by High-Mass MALDI mass Spectrometry. Jerome Haustant, Bice Bruppacher, Ryan Wenzel, Alexis Nazabal