Based on our unique MALDI mass spectrometry technology and decades of crosslinking experiences, CovalX offers specialized characterization of epitopes with high resolution. Using mass spectrometry and covalent tagging (though crosslinking), it is possible to characterize conformational and linear epitopes in weeks with a resolution of 1-3 amino acids. Without the need for peptide synthesis or micro-array chips, mass spectrometry allows identity of the interacting peptides with unmatched accuracy. This technique provides the lowest cost, lowest sample consumption and shortest analysis time possible when compared with other conformational epitope mapping techniques. Additionally, because the protein complexes are first covalently stabilized, multiple enzymes can be utilized providing highly optimized digestion conditions with the highest sequence coverage possible.
This technique can provide both epitope and paratope data in one experiment. Over the years this technique has been established as a reliable source of data for both research and patent filing purposes.
An overview of epitope mapping technologies can be seen here.