The analysis of protein complexes by MALDI mass spectrometry is typically labor intensive because of the tendency of these complexes to dissociate during analysis. CovalX has developed a method for the easy and fast analysis of protein complexes using High-Mass MALDI mass spectrometry. The first step of the analysis is to stabilize the noncovalent complexes of interest using dedicated cross-linking reagents and protocols. After stabilization, the sample is ready for direct analysis by High-Mass ToF mass spectrometry.
To analyze intact protein complexes by High-Mass MALDI ToF mass spectrometry, it is crucial to stabilizing specifically the complexes with highly efficient cross-linking reagents. CovalX has developed dedicated reagent and buffers to prepare non-covalent protein complexes for High-Mass MALDI analysis. To increase cross-linking efficiency, CovalX's reagents contain cocktails of cross-linkers with different spacers lengths able to covalently bind specific protein complexes with the highest efficiency. The specificity of CovalX cross-linking reagents allows you to covalently stabilize linked protein complexes even in contaminated or unpurified samples.
|MALDI MS Analysis Kits and Reagents for protein interactions||Mass Range||Part No|
|K50 MALDI MS Analysis Kit||0-50 kDa||2008k50|
|K100 MALDI MS Analysis Kit||20-100 kDa||2008k100|
|K200 MALDI MS Analysis Kit||100-1000kDa||2008k200|
|R50 MALDI MS Analysis reagent||0-50 kDa||2008R50|
|R100 MALDI MS Analysis reagent||20-100 kDa||2008R100|
|R200 MALDI MS Analysis reagent||100-1000kDa||2008R200|
|Calibration Kits C150||Calibration 0-150 kDa||2008c150|
|Calibration kits C450||Calibration 0-450 kDa||2008c450|