CovalX De Novo Sequencing Brochure
This website uses cookies so that we can provide you with the best user experience possible. Cookie information is stored in your browser and performs functions such as recognising you when you return to our website and helping our team to understand which sections of the website you find most interesting and useful.
Expert De Novo Sequencing Solutions for Antibodies and Proteins
Talk to Our Scientists
Protein/Peptide De Novo Sequencing by Mass Spectrometry
The principle of de novo sequencing involves determining the sequence of nucleotides or amino acids without relying on a reference sequence. In DNA, “de novo” refers to the assembly of a genome from raw sequencing data without using a reference genome for alignment or mapping. In protein sequencing, it involves interpreting Mass Spectrometry (MS) data from fragmented peptides to deduce the amino acid sequence without a reference protein. Please be aware that CovalX is a specialized proteomics CDMO, focused exclusively on protein analysis. We do not provide DNA or RNA sequencing services.
The Principle of De Novo Sequencing
De novo sequencing is the process of determining the amino acid sequence of a protein-based directly on the fragment ions detected in the mass spectrometer. After sample receipt, the material is purified or concentrated as needed. The protein is then digested using up to four selected multi-enzymes to ensure the highest possible sequence coverage. The proteins are then introduced into mass spectrometer after nano-flow liquid chromatography separation. Using special algorithms, our analysis software can interpret and assign the amino acid sequences without any prior knowledge from the protein.
Sample requirements: 100-400 μg of recombinant proteins, purified proteins
Data turnaround: 1-2 weeks
Deliverable: The protein sequence
Understanding De Novo Assembly vs. Reference Genomes
Understanding the amino acid sequence of peptides derived from protein digestion is essential for comprehending the biological role of the protein. MS techniques, particularly tandem mass spectrometry (MS/MS), are pivotal in de novo sequencing of antibodies and peptides, enabling the direct deduction of sequence information from experimental data. This empowers researchers to uncover novel insights into the structure, function, and interactions of biological molecules.
Our Expertise
Our high-resolution nano-LC coupled with the ultra high-resolution mass spectrometry detection is finely tuned to produce unmatched quality in antibody sequencing. With over 15 years of experience in the field of biotherapeutics, our scientists have access to a full range of the latest mass spectrometry equipment and software, ensuring the fastest and most accurate results for our clients.