Protein-Protein Interaction Analysis with High-Mass MALDI Mass Spectrometry
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What is the best assay for protein-protein interaction?
At CovalX, we have developed and optimized a state-of-the-art method for fast, sensitive, and accurate protein complex analysis using High-Mass MALDI mass spectrometry. Our approach allows the detection of unfragmented and undigested protein complexes intact, leveraging specially developed cross-linking reagents and a high-mass detection system. This method requires no sample pre-labeling, tagging, or immobilization, as samples are measured directly in solution.
Advanced Protein Complex Analysis
Our technique employs cross-linking chemistry and High-Mass MALDI ToF mass spectrometry to analyze non-covalent protein complexes within the 1-2000 kDa range. This versatile method can handle simple aggregate multimers (dimers, trimers, etc.), antibody fragments (Fab: one-to-one), monoclonals (two-to-one), and advanced stoichiometries of nearly any protein-protein interaction.
Advantages of High-Mass MALDI for Protein Interactions
- Speed: Complete assays in just 30 minutes.
- Cost-Effective: More affordable than traditional protein interaction techniques.
- Accuracy: Provides precise protein-protein interaction data on the stoichiometry and partners involved in protein complexes.
- Label-Free: No need for immobilization or labeling.
- Sensitivity: Requires only a few hundred femtomoles of the protein sample.
Expertise in Protein-Protein Interaction Analysis
CovalX is dedicated to providing the best assays for protein-protein interaction analysis. Our innovative methods and technologies offer unparalleled insights into protein interactions, from protein-RNA interactions to protein-ligand interactions and protein-antibody interactions. Contact us to learn more about our services and how we can support your research and development needs.
CovalX Complex Analysis