Cross-Linking Reagents for Molecular Interaction Analysis



Claudia Bich, Alexis Nazabal, Ryan Wenzel, and Renato Zenobi


  1. Eidgenoessische Technische Hochschule Zurich (ETHZ), Covalx AG


The invention describes the use of particular cross-linking reagents containing 1-hydroxybenzotriazole or 1-hydroxy-7-azabenzotriazole groups as reactive groups for cross-linking reactions of supramolecular target-ligand-complexes. The resulting cross-linked products may be directly analyzed using mass spectrometry, gel or fluorescence based technologies, X-ray crystallography, NMR or other analytical technologies. The method using High-Mass MALDI mass spectrometry provides various biological applications such as characterization of antibodies, drug discovery, and protein complex analysis including automated or higher throughput applications.

CovalX Technology Referenced

XL Kits

Patent Number

EP2435464 B1

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Categories : Patents